Update app.py

app.py

@@ -173,7 +173,8 @@ with ui.navset_card_tab(id="tab"):
             import seaborn as sns 
             plot_type = input.plot_type_distro_sub()
             if plot_type == "2D sub-specie - nominal":
-                df = process_data_sub_specie(MASTER_DF, input.virus_selector_2(), input.variance())
+                df = pd.read_parquet('virus_ds.parquet')
+                df = process_data_sub_specie(df, input.virus_selector_2(), input.variance())
                 df = df.explode('two_d').copy()
                 df['x'] = np.array((df['two_d'].tolist()))[:,0]
                 df['y'] = np.array((df['two_d'].tolist()))[:,1]